DENARASE is a highly active endonuclease from Serratia marcescens. This enzyme is widely used in biopharmaceutical manufacturing to quantitatively eliminate host cell DNA and RNA during the production of biologicals and vaccines, as well as for viscosity reduction in biotechnology applications.
DENARASE has a high purity of ≥ 99 % and is manufactured in full compliance with GMP requirements, without the use of antibiotics and only with raw materials of non-animal origin.
The patent-protected production process is based on the recombinant expression in a Bacillus sp., combining high product yields with the advantages of an endotoxin-free production strain. DENARASE meets all relevant safety and quality requirements for the use in regulated fields.
Please use the inquiry form below or contact us via DENARASE@c-LEcta.com to find out what c-LEcta‘s DENARASE can do for your bioprocess demands or to request your individual quotation.
DENARASE is a proprietary product of c-LEcta GmbH, Germany. For more information about the company please visit our corporate website:
Activity: >250 U/μl
Purity: ≥ 99%
Production host: Bacillus sp.
Full compliance with GMP
Manufacturing free of products of animal origin
Endotoxin-free production strain
Form: buffered aqueous glycerol solution
Storage Temperature: -20°C
Application & Benefits
Successful applications of DENARASE include the purification of vaccines, proteins expressed in inclusion bodies and monoclonal antibodies.
Residual DNA in bioprocesses can cause high viscosity and often interacts with the target product, resulting in lower recovery rates.
To meet regulatory requirements, residual DNA must be removed from biotech products. DENARASE breaks DNA chains into fragments of 2–5 bases, making it an efficient DNA removal technology.
DNA can bind to target products such as monoclonal antibodies and viruses. Blocking of functional groups needed for affinity may result in lower recovery rates after chromatography steps. DENARASE reduces the DNA content at an early stage of the process, thus increasing product yields.
High DNA concentrations cause high viscosities, rendering filtration and chromatography difficult. DENARASE helps to reduce viscosity and to improve process economics.
DENARASE can prevent cell clumping in bioreactors.
DENARASE is the first choice for pharmaceutical manufacturers to optimize their process efficiencies and improve product recovery rates.
The protein consists of two subunits with a calculated molecular weight of 27 kDa each. DENARASE unspecifically cleaves all forms of DNA and RNA very efficiently. It hydrolyses phosphodiester bonds between nucleotides (single-stranded, double stranded, linear, and circular, sequence-independent) leaving short fragments with a length of 2-5 bases with a 5’-monophosphate end.
DENARASE is active and stable in all tested commonly used buffers, even in the presence of ionic detergents.
For more technical information on DENARASE, please see the Product Information Sheet or Validation Guide (Download Section).
|DENARASE, 1 MU||20804-1M||1 MU |
|≥ 99%||> 250 U/µl||on request|
|DENARASE, 5 MU||20804-5M||5 MU |
|≥ 99%||> 250 U/µl||on request|
|DENARASE, 100 kU||20804-100k||100 kU |
|≥ 99%||> 250 U/µl||325,00 €|
|DENARASE, 500 kU||20804-500k||500 kU |
|≥ 99%||> 250 U/µl||990,00 €|
The products DENARASE, 1 MU and DENARASE, 5 MU (Article Number: 20804-1M, 20804-5M) are produced and filled under GMP and are suitable for biopharmaceutical manufacturing applications.
The products DENARASE, 100 kU and DENARASE, 500 kU (Article Number: 20804-100k, 20804-500k) for biotechnology applications are produced under GMP and filled under ISO 9001.
Please use the inquiry form below or contact us via DENARASE@c-LEcta.com to find out what c-LEcta's DENARASE® can do for your bioprocess demands.
Which quantity of DENARASE do I need for my application?
The amount of DENARASE or the incubation time that may be required depends on the nucleic acid content and/or further process conditions (e.g. temperature, pH and concentrations of cofactor and inhibitors) which may differ from process to process. Therefore, the optimal quantity of DENARASE has to be determined individually.
What do I have to consider for a well-performing application of DENARASE?
DENARASE is a very robust enzyme that is active under a broad range of conditions. Similarly to other enzymes, its activity depends on various parameters like temperature, pH and concentrations of cofactor and inhibitors. For more detailed information please see the Product Information Sheet or Validation Guide (Download Section).
How can I remove DENARASE from my process?
There are several different options for the efficient removal of DENARASE from target products during downstream purification. The two main strategies are as follows:
In case the target product is purified via specific capturing (e.g. affinity chromatography), DENARASE will remain in the flow-through and be eliminated together with other undesired impurities. Depending on the process, anion exchange, cation exchange, hydrophobic interaction, hydroxyapatite and or size exclusion might be applied.
If it is necessary to specifically remove DENARASE, anion exchange resins or hydrophobic interaction chromatography media can be used. Furthermore, DENARASE can be captured by affinity chromatography media mimicking DNA or nucleotides (e.g. Cibacron Blue 3G resins). Alternatively, tangential flow filtration (TFF) may also be applied for specific removal of DENARASE from process solutions. Depending on the target product and the overall process, the appropriate type of chromatography or filtration method can be selected.
How can I determine residual traces of DENARASE after a (chromatographic) removal step in my process?
Residual traces of DENARASE can be reliably determined via commercially available ELISA kits.
Downloads & Publications
Validation Guide (on request)
Customer Comments / Feedback
„We are more than happy with the excellent activity of DENARASE”
(German Biotech Company, Viral Vector production, Head of USP development)
„DENARASE worked better compared to other nuclease samples, depending on the samples DNA concentration.”
(Biotech Company, Finland, Vector process development, Senior Scientist)
„DENARASE is an important ingredient in our current up-scale cleaning process, of our whole DSP concept.”
(German Biotech Company, Manager Process & Assay development)
„DENARASE is comparable to our standard nuclease, therefore a real alternative for us.”
(German Biotech Company, Head of Analytic development/QC)
„Yes, we successfully tested DENARASE and it worked at least as well as our standard nuclease. Because of the competitive pricing we already ordered.”
„Yes, for future incoming projects we will use DENARASE.”
(Canadian pharmaceutical manufacturer, Lead Scientist)
„We are going to use DENARASE exclusively and no longer our usual nuclease.
The product is excellent in performance and price.”
(US biopharmaceutical manufacturer, Lab Manager Vector Production)
„We are mainly interested in DENARASE to remove nucleic acid contaminants from bacterial hosts when growing them at large scale.
We have been using another nuclease, but have not been pleased with its efficiency.
DENARASE is performing excellent and we are purchasing it.”
(US clinical-stage biotechnology company, Scientist)
„We have used DENARASE in our manufacturing production. It is good according to our test.
We plan to use it in next valuable schedule.”
(US-Children Hospital, Clinical Vector Core, Lead Scientist)
„I tested the DENARASE yesterday and it was equally good as our standard in my setting.
I will continue using it in variety of conditions that I normally use and I will most certainly be purchasing my future enzymes from you.”
* All marked fields are mandatory fields.
A confirmation email will be sent upon inquiry. Feedback will be usually given in a two working days period.